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Retinoblastoma Protein (Rb) antibody (N-Term)

The Rabbit Polyclonal anti-Retinoblastoma Protein (Rb) antibody has been validated for WB. It is suitable to detect Retinoblastoma Protein (Rb) in samples from Human, Mouse and Monkey.
Catalog No. ABIN6745232

Quick Overview for Retinoblastoma Protein (Rb) antibody (N-Term) (ABIN6745232)

Target

See all Retinoblastoma Protein (Rb) (Rb Protein) Antibodies
Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reactivity

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  • 51
  • 37
  • 8
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  • 3
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Human, Mouse, Monkey

Host

  • 123
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Rabbit

Clonality

  • 121
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Polyclonal

Conjugate

  • 86
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This Retinoblastoma Protein (Rb) antibody is un-conjugated

Application

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Western Blotting (WB)
  • Binding Specificity

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    N-Term

    Specificity

    Human RB1

    Predicted Reactivity

    Percent identity by BLAST analysis: Human (100%) Pig (93%) Bovine, Rabbit (92%) Mouse (80%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide from N-Terminus of human RB1 (P06400, NP_000312). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Pig (93%), Galago, Bovine, Rabbit, Drosophila (92%), Panda (85%), Marmoset (82%), Mouse (80%), Rat (78%).

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

    Alternative Name

    RB1 / Retinoblastoma / RB

    Background

    Name/Gene ID: RB1

    Synonyms: RB1, p105-Rb, Osteosarcoma, PRb, Retinoblastoma, Retinoblastoma 1, OSRC, RB, Pp110

    Gene ID

    5925

    NCBI Accession

    NP_000312

    UniProt

    P06400

    Pathways

    Mitotic G1-G1/S Phases
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