Heme Oxygenase 1, Heme Oxygenase 2 (AA 101-200) antibody

Catalog No. ABIN677273

Product Details

Target: Heme Oxygenase 1, Heme Oxygenase 2

Binding Specificity: AA 101-200

Reactivity: Chicken, Human, Mouse, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Chicken, Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Sheep,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Heme Oxygenase 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Zeng, Li, Zhang, Liu, Li, Xiong, He, Wang, Ye: "Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity." in: Transplantation proceedings, Vol. 49, Issue 2, pp. 366-372, (2017) (PubMed).

Wang, Gu, Liu, Bai, Liang, Wang: "TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 4310475, (2017) (PubMed).

Maïga, Allain, Hauet, Roumy, Baulier, Scepi, Dierick, Van Hoorebeke, Hannaert, Guy, Favreau: "Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model." in: PLoS ONE, Vol. 12, Issue 7, pp. e0181067, (2017) (PubMed).

Bai, Yu, Liu, Wang, Jing, Li, Zhang, Huo, Li, Gao, Qi, Kang: "Central administration of tert-butylhydroquinone attenuates hypertension via regulating Nrf2 signaling in the hypothalamic paraventricular nucleus of hypertensive rats." in: Toxicology and applied pharmacology, Vol. 333, pp. 100-109, (2017) (PubMed).

Xuan, Niu, Chen: "Astaxanthin blocks preeclampsia progression by suppressing oxidative stress and inflammation." in: Molecular medicine reports, Vol. 14, Issue 3, pp. 2697-704, (2017) (PubMed).

Wang, Gu, Liu, Bai, Wang: "Fluoxetine protects against methamphetamine‑induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats." in: Molecular medicine reports, Vol. 15, Issue 2, pp. 673-680, (2016) (PubMed).

Wei, Li, Ji, Hu, Ji: "The cardioprotective effect of sodium tanshinone IIA sulfonate and the optimizing of therapeutic time window in myocardial ischemia/reperfusion injury in rats." in: Atherosclerosis, Vol. 235, Issue 2, pp. 318-27, (2015) (PubMed).

Song, Zukor, Liberman, Kaduri, Arvanitakis, Bennett, Schipper: "Astroglial heme oxygenase-1 and the origin of corpora amylacea in aging and degenerating neural tissues." in: Experimental neurology, Vol. 254, pp. 78-89, (2014) (PubMed).

Dey, Chang, Wainwright, Ahmed, Han, Balyasnikova, Lesniak: "Heme oxygenase-1 protects regulatory T cells from hypoxia-induced cellular stress in an experimental mouse brain tumor model." in: Journal of neuroimmunology, Vol. 266, Issue 1-2, pp. 33-42, (2014) (PubMed).

Lee, Hsu, Hsu, Pan: "Dimerumic acid attenuates receptor for advanced glycation endproducts signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promotes methylglyoxal metabolism into d-lactic acid." in: Free radical biology & medicine, Vol. 60, pp. 7-16, (2013) (PubMed).

Wei, You, Ling, Wei, Wang, Li, Chen, Du, Ji et al.: "Regulation of antioxidant system, lipids and fatty acid ?-oxidation contributes to the cardioprotective effect of sodium tanshinone IIA sulphonate in isoproterenol-induced myocardial infarction in ..." in: Atherosclerosis, Vol. 230, Issue 1, pp. 148-56, (2013) (PubMed).

Target Details

Target: Heme Oxygenase 1, Heme Oxygenase 2

Background:

Synonyms: HO-1, HSP32, HMOX1D, bK286B1, HMOX1, HO, HO1

Background: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Gene ID: 3162

UniProt: P09601