TNF alpha antibody (AA 77-232)

Catalog No. ABIN6783661

The Mouse Monoclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Pig. It has been validated for WB.

Quick Overview for TNF alpha antibody (AA 77-232) (ABIN6783661)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TNF alpha Antibody

Binding Specificity: AA 77-232

Specificity: The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant TNFa (Leu77-Leu232) expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:100 - 1:400)

Usage: ICC 5-30 μg/mL, WB 0.5-5 μg/mL,

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.02 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Aliquot and store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome