Ephrin B antibody (pSer300)
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- Target See all Ephrin B (EphB) products
- Ephrin B (EphB)
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Binding Specificity
- pSer300
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Reactivity
- Human, Mouse, Chicken, Rat, Cow, Dog, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Ephrin B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Cow, Human, Mouse, Rat
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Ser300
- Isotype
- IgG
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anti-Ephrin B (EphB) (pTyr324), (pTyr329) antibody
EphB Reactivity: Human WB, IF (p), IF (cc), ELISA, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Ephrin B (EphB) (AA 566-615) antibodyEphB Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB (1:100-500),IHC-P (1:100-500),IF (p) (1:100-500)
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C for 12 months.
- Expiry Date
- 12 months
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- Target
- Ephrin B (EphB)
- Abstract
- EphB Products
- Background
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Synonyms: Ephrin B phospho Y330, p-Ephrin B phospho Y330, CEK5 ligand, EFNB1, ELK L, EphrinB, Ephrin-B, ELK ligand, ELKL, EPH related receptor tyrosine kinase ligand 2, Ephrin B Precursor, Ephrin B1, Ephrin B2, LERK 2, LERK2, EFNB2_HUMAN.
Background: Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
- Molecular Weight
- 38kDa
- Gene ID
- 1947
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