Ephrin B antibody (pSer300) (Cy7)

Catalog No. ABIN684544

Product Details anti-Ephrin B Antibody (Cy7)

Target: Ephrin B (EphB)

Binding Specificity: pSer300

Reactivity: Human, Mouse, Chicken, Rat, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B antibody is conjugated to Cy7

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Ser300

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Ephrin B

Target: Ephrin B (EphB)

Abstract: EphB Products

Background:

Synonyms: Ephrin B phospho Y330, p-Ephrin B phospho Y330, CEK5 ligand, EFNB1, ELK L, EphrinB, Ephrin-B, ELK ligand, ELKL, EPH related receptor tyrosine kinase ligand 2, Ephrin B Precursor, Ephrin B1, Ephrin B2, LERK 2, LERK2, EFNB2_HUMAN.

Background: Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.

Molecular Weight: 38kDa

Gene ID: 1947