Srebp-1/2 (AA 301-450) antibody (HRP)
-
- Target
- Srebp-1/2
- Binding Specificity
- AA 301-450
- Reactivity
- Cow, Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- HRP
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Cow, Human, Mouse, Rat
- Predicted Reactivity
- Sheep,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human SREBP-1
- Isotype
- IgG
-
-
- Application Notes
-
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- Srebp-1/2
- Background
-
Synonyms: Sterol regulatory element binding protein 1, ADD 1, BHLHD1, D630008H06, SREBF 1, SREBF1, SREBP 1, SREBP1, SREBP 1c, Sterol regulatory element binding protein 1, Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1, Sterol regulatory element binding transcription factor 1.
Background: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Isoform SREBP-1A is much more active than isoform SREBP-1C in stimulating transcription from SRE-1-containing promoters. [SUBUNIT] Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. [SUBCELLULAR LOCATION] Endoplasmic reticulum membrane, Multi-pass membrane protein. Golgi apparatus membrane, Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane, Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. [SUBCELLULAR LOCATION] Processed sterol regulatory element-binding protein 1: Nucleus. Belongs to the SREBP family.
- Gene ID
- 6720, 6721
-