CK8 & CK18 antibody
Reactivity: Human
FACS, IF, IHC (f)
Host: Mouse
Monoclonal
5D3
unconjugated
-
- Target
- CK8 & CK18
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
- Cross-Reactivity (Details)
- Human.
- Immunogen
- Cytoskeleton preparation from human breast cancer MCF-7 cells
- Clone
- 5D3
- Isotype
- IgG1 kappa
-
- Application Notes
-
Known_Application: Flow Cytometry (2-5 µL/million cells), Immunofluorescence (1:100-1:200), Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.
- Restrictions
- For Research Use only
-
- Buffer
- Tissue culture supernatant with 0.05% Azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- CK8 & CK18
- Background
-
CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 8/18
Cellular localisation: Cytoplasmic - Molecular Weight
- 52.5kDa (CK8), 45kDa (CK18)
- Gene ID
- 3856, 3875, 533782
- UniProt
- P05787, P05783
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