KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18 antibody

Catalog No. ABIN6933727

Product Details

Target: KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18

Reactivity: Human, Marmoset, Mouse, Pig, Cow, Rat, Frog, Goat, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Purification: Purified by Protein A/G

Immunogen: Keratin-enriched preparation from cultured human A431

Clone: C11

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa, A431 cells, Skin, Colon carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-4 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18

Gene ID: 3851, 3852, 3853

UniProt: P19013, P13647, P02538, P05787, P13645, P13646, P05783