KRT6A/KRT6B/KRT6C (C-Term) antibody

Catalog No. ABIN6933936

Product Details

Target: KRT6A/KRT6B/KRT6C

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunostaining (ISt)

Specificity: This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Purification: Purified by Protein A/G

Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6

Clone: SPM269

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: RAW, HeLa, Suprabasal cells of the outer hair follicle root sheath in skin. Basal cell carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin (Sigma Cat. #P-7012) at 1 mg/mL Tris-HCl, pH 2.0 for 10-15 min at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: KRT6A/KRT6B/KRT6C

Alternative Name: KRT6A, KRT6B, KRT6C

Molecular Weight: 56kDa

Gene ID: 3853, 3854, 286887

UniProt: P02538, P04259, P48668