beta-2 Microglobulin antibody

Catalog No. ABIN6936450

The Mouse Monoclonal anti-beta-2 Microglobulin antibody is suitable to detect beta-2 Microglobulin in samples from Human and Non-Human Primate. It has been validated for WB, IHC, FACS and IF.

Quick Overview for beta-2 Microglobulin antibody (ABIN6936450)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BBM-1

Product Details anti-beta-2 Microglobulin Antibody

Purpose: Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody

Specificity: Recognizes a protein of 12 kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Cross-Reactivity (Details): Human. Non-Human primates.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: MOLT-4 human T cell line

Isotype: IgG2b, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-4 μg/mL), Immunohistochemistry (Frozen) (1-2 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Positive_Control: THP-1, Raji, HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M

Background:

B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p

Human cervix, melanoma, lymphoma, endometrium, carcinoma of stomach, kidney or colon. melanomas or lymphoma. Raji, THP-1, HL-60 or HeLa cells.

Molecular Weight: 12kDa

Gene ID: 567, 534255

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process