Recombinant Caldesmon antibody
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- Target See all Caldesmon (CALD1) Antibodies
- Caldesmon (CALD1) (Caldesmon 1 (CALD1))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Caldesmon antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
- Cross-Reactivity (Details)
- Human and Rat. Others not known.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human full-length CALD1 protein
- Clone
- CALD1-1424R
- Isotype
- IgG
- Top Product
- Discover our top product CALD1 Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), ,Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Ovary, Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Caldesmon (CALD1) (Caldesmon 1 (CALD1))
- Alternative Name
- CALD1 (CALD1 Products)
- Synonyms
- CDM antibody, H-CAD antibody, HCAD antibody, L-CAD antibody, LCAD antibody, NAG22 antibody, l-Cad antibody, 4833423D12Rik antibody, AI195384 antibody, AV071549 antibody, AW536160 antibody, C920027I18Rik antibody, CALD1 antibody, Caldesmon antibody, caldesmon 1 antibody, CALD1 antibody, Cald1 antibody
- Background
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CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22,Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic - Molecular Weight
- 150kDa
- Gene ID
- 800, 490203
- UniProt
- Q05682
- Pathways
- Myometrial Relaxation and Contraction
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