CD3 epsilon antibody (Cytoplasmic Domain)
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- Target See all CD3 epsilon (CD3E) Antibodies
- CD3 epsilon (CD3E)
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Binding Specificity
- AA 156-168, Cytoplasmic Domain
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD3 epsilon antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
- Cross-Reactivity (Details)
- Human, Rat, Guinea pig, Rabbit, Pig, Horse, Cow and Chicken. Others not known.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain
- Clone
- PC3-188A
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD3E Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blotting (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Tonsil and lymph node
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3E (CD3E Products)
- Synonyms
- cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
- Background
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CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3e (T-Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic - Molecular Weight
- 20kDa
- Gene ID
- 916, 3003
- UniProt
- P07766
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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