CD47 antibody (AA 18-135)

Catalog No. ABIN6936742

This anti-CD47 antibody is a Mouse Monoclonal antibody detecting CD47 in FACS, IHC, IF, ELISA, IHC (f) and Coat. Suitable for Human.

Quick Overview for CD47 antibody (AA 18-135) (ABIN6936742)

Target: CD47

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: CD47-3019

Product Details anti-CD47 Antibody

Binding Specificity: AA 18-135

Specificity: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen,CD47 / IAP (Integrin Associated Protein)
Cellular localisation: Cell Surface

Molecular Weight: ~50kDa

Gene ID: 961, 446414

UniProt: Q08722