CD55 antibody

Catalog No. ABIN6936757

Product Details anti-CD55 Antibody

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), DNA Microscopy (DNA Mic), DNA Microscopy (DNA Mic)

Specificity: Recognizes a single chain glycoprotein of 70 kDa, identified as CD55. This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human umbilical vein endothelial cells (HUVEC)

Clone: F4-29D9

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, HUT-78, K562, YT, U937, MG63 and human lymphocytes. Human lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 (CD55 Products)

Synonyms: CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody

Background: CD55, CD55 Cromer blood group system, Complement decay-accelerating factor, CROM, DAF, Decay Accelerating Factor for Complement, Decay accelerating factor GPI-form, Decay accelerating factor soluble-form, GPI-DAF,CD55 / Decay Accelerating Factor (DAF)
Cellular localisation: Cell Surface

Molecular Weight: 70kDa

Gene ID: 1604, 126517

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity