CYP1A1/2 antibody
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- Target See all CYP1A1/2 Antibodies
- CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CYP1A1/2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies, the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.
- Cross-Reactivity (Details)
- Human. Mouse. Rat. Primate.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- 3-methylcholanthrene induced rat cytochrome P450 protein.
- Clone
- MC1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CYP1A1/2 Primary Antibody
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- Application Notes
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Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Liver or Cardiac muscle.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))
- Alternative Name
- CYP1A1 / CYP1A2 (CYP1A1/2 Products)
- Synonyms
- CP12 antibody, Cyp1a1 antibody, P450-3 antibody, cytochrome P450, family 1, subfamily a, polypeptide 2 antibody, Cyp1a2 antibody
- Background
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Aryl hydrocarbon hydroxylase, CP11, CP12, CYP1A1, CYP1A2, CYPIA1, CYPIA2, Cytochrome P450 family 1 subfamily A polypeptide 1, Cytochrome P450 family 1 subfamily A polypeptide 2, P1 450, P3 450, P450 form 4, P450 form 6, P450 P1, P450 P3,Cytochrome P450 1A1/1A2
Cellular localisation: Cytoplasmic - Molecular Weight
- 58kDa
- Gene ID
- 1543, 1544, 437060
- UniProt
- P04798, P05177
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