FCER2 antibody (AA 48-321)

Catalog No. ABIN6937218

Product Details anti-FCER2 Antibody

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Binding Specificity: AA 48-321

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCER2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: CD23 (FCE2) is a type II integral membrane glycoprotein that is expressed on mature B cells, monocytes, eosinophils, platelets and dendritic cells. CD23 is a low affinity IgE receptor that mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. CD23 associates as an oligomer where cooperative binding of at least two lectin domains is required for high affinity IgE binding to CD23. It may play a role in antigen presentation by B cells by interacting with CD40. CD23 has been shown to be associated with the Fyn tyrosine kinase. The truncated molecule can be secreted, then function as a potent mitogenic growth factor. CD23 is expressed on a subpopulation of peripheral blood cells, B-lymphocytes and on EBV transformed B lymphoblastoid cell lines. CD23 is also detected in neoplastic cells from cases of B cell chronic lymphocytic leukemia and some cases on centroblastic/centrocytic lymphoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein (exact sequence is proprietary)

Clone: FCER2-3592

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human lymph node or tonsil (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FCER2

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Alternative Name: FCER2 (FCER2 Products)

Synonyms: BLAST-2 antibody, CD23 antibody, CD23A antibody, CLEC4J antibody, FCE2 antibody, IGEBF antibody, Fcer2a antibody, Fc fragment of IgE receptor II antibody, FCER2 antibody, Fcer2 antibody

Background: BLAST-2, C-type lectin domain family 4, member J, Fc fragment of IgE low affinity II receptor, Fc-epsilon-RII, FCER2, FCER2A, FceRII, IgE-binding factor (IGEBF), Immunoglobulin E receptor, low affinity II, Lymphocyte IgE receptor,CD23 (Fc Epsilon RII)
Cellular localisation: Cell Surface

Molecular Weight: 37kDa (soluble form), 45kDa (membrane-bound form)

Gene ID: 2208, 465778

UniProt: P06734

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process