FCGR2A antibody

Catalog No. ABIN6937220

Product Details anti-FCGR2A Antibody

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR2A antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Functional Studies (Func)

Specificity: This MAb binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human FCGR2A protein

Clone: FCGR2A-479

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: K-562 cells. Lymph node, tonsil and placenta.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FCGR2A

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Alternative Name: FCGR2A (FCGR2A Products)

Synonyms: CD32 antibody, CD32A antibody, CDw32 antibody, FCG2 antibody, FCGR2 antibody, FCGR2A1 antibody, FcGR antibody, IGFR2 antibody, CD32a antibody, Fc fragment of IgG receptor IIa antibody, FCGR2A antibody

Background: CD32A, Fc fragment of IgG low affinity IIa receptor, Fc gamma RIIa, FCGR2A, FcgRII, IGFR2, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a, Ly-17, Ly-m20, LyM-1, Lymphocyte antigen 17,CD32 (Fc Gamma RIIa)
Cellular localisation: Cell Surface

Molecular Weight: 40kDa

Gene ID: 2212, 352642

UniProt: P12318