GAD antibody (AA 72-135)
-
- Target See all GAD (GAD1) Antibodies
- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
-
Binding Specificity
- AA 72-135
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This GAD antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)
- Clone
- GAD1-2391
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product GAD1 Primary Antibody
-
-
- Application Notes
-
Known_Application: ELISA (For coating, order antibody without BSA),Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K-562 or HEK293 cells. Pancreas.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
-
- Target
- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
- Alternative Name
- GAD1 (GAD1 Products)
- Synonyms
- CPSQ1 antibody, GAD antibody, SCP antibody, GAD-67 antibody, cpsq1 antibody, gad1 antibody, gad1-A antibody, gad67 antibody, GAD67 antibody, GAD1 antibody, GLUTAMATE DECARBOXYLASE antibody, GLUTAMATE DECARBOXYLASE 1 antibody, MKP11.30 antibody, MKP11_30 antibody, glutamate decarboxylase antibody, EP10 antibody, GAD25 antibody, GAD44 antibody, Gad-1 antibody, Gad67 antibody, glutamate decarboxylase 1 antibody, glutamate decarboxylase 1 L homeolog antibody, glutamate decarboxylase antibody, glutamate decarboxylase 1b antibody, GAD1 antibody, gad1.1.L antibody, GAD antibody, Gad1 antibody, gad1b antibody
- Background
-
67 kDa glutamic acid decarboxylase, CPSQ1, DCE1, GAD67, GAD1, Glutamate decarboxylase 1, SCP,GAD1 / GAD67 (GABAergic Neuronal Marker)
Cellular localisation: Cytoplasmic - Molecular Weight
- 67kDa
- Gene ID
- 2571, 420036
- UniProt
- Q99259
-