Recombinant GP2 antibody (AA 35-179)

Catalog No. ABIN6937336

Product Details anti-GP2 Antibody

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 35-179

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GP2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Human. Does not react with Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human GP2 protein fragment (around aa35-179) (exact sequence is proprietary)

Clone: GP2-2569R

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PANC-1 cells. Pancreas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GP2

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Alternative Name: GP2 (GP2 Products)

Synonyms: ZAP75 antibody, 2310037I18Rik antibody, AV060639 antibody, gp80 antibody, glycoprotein 2 antibody, glycoprotein 2 (zymogen granule membrane) antibody, GP2 antibody, Gp2 antibody

Target Type: Viral Protein

Background: Glycoprotein 2 (zymogen granule membrane), GP2, Pancreatic zymogen granule membrane associated protein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75,GP2 (Glycoprotein 2) / ZAP75
Cellular localisation: Cell Surface and Secreted

Molecular Weight: 59kDa

Gene ID: 2813, 53985

UniProt: P55259