GPI antibody

Catalog No. ABIN6937345

Product Details anti-GPI Antibody

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GPI antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length GPI protein

Clone: CPTC-GPI-1

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T47D, PC3, MCF-7, HepG2 and A549 cells. Brain, pancreas or thyroid carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: GPI (GPI Products)

Synonyms: PGI antibody, Amf antibody, Gpi1 antibody, GPI antibody, DDBDRAFT_0185620 antibody, DDBDRAFT_0231026 antibody, DDB_0185620 antibody, DDB_0231026 antibody, CG8251 antibody, Dmel\\CG8251 antibody, Gpi antibody, pgi antibody, Gpi-1 antibody, Gpi-1r antibody, Gpi-1s antibody, Gpi-1t antibody, Gpi1-r antibody, Gpi1-s antibody, Gpi1-t antibody, Gpi1s antibody, MF antibody, NK antibody, NK/GPI antibody, Org antibody, AMF antibody, GNPI antibody, NLK antibody, PHI antibody, SA-36 antibody, SA36 antibody, gpi antibody, pgi-2 antibody, glucose-6-phosphate isomerase antibody, gpi antibody, glucose-6-phosphate isomerase 1 antibody, gpi Glucose-6-phosphate isomerase antibody, Phosphoglucose isomerase antibody, glucose phosphate isomerase 1 antibody, glucose-6-phosphate isomerase b antibody, GPI antibody, Gpi antibody, gpi antibody, pgi antibody, GPI1 antibody, Pgi antibody, Gpi1 antibody, gpib antibody

Target Type: Viral Protein

Background: AMF, aurocrine motility factor, EC 5.2.1.9, GNPI, Gpi1, hexose monophosphate isomerase, neuroleukin, NLK, oxoisomerase, PGI, PHI, phosphoglucose isomerase, phosphosaccharomutase, SA-36, sperm antigen 36,Glucose 6-Phosphate Isomerase
Cellular localisation: Cytoplasm. Secreted.

Molecular Weight: 55kDa

Gene ID: 2821, 466471

UniProt: P06744