HLA-A antibody

Catalog No. ABIN6937389

Product Details anti-HLA-A Antibody

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of the 1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Normal human peripheral blood lymphocytes

Clone: 108-2C5

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Raji, THP-1 and Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Alternative Name: HLA-A (HLA-A Products)

Synonyms: HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody

Background: HLA-A, HLA class 1A, MHC class I HLA-A,HLA-A (MHC I)
Cellular localisation: Cell Surface

Molecular Weight: ~41kDa

Gene ID: 3105, 181244, 654404

UniProt: P01889, P30443, P30499

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints