HLA-A antibody
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- Target See all HLA-A Antibodies
- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HLA-A antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10
- Clone
- CATA-1
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product HLA-A Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: THP-1 and Jurkat cells. Tonsil or lymph node.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
- Alternative Name
- HLA-A (HLA-A Products)
- Synonyms
- HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody
- Background
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HLA-A, HLA class 1A, MHC class I HLA-A,HLA-Aw32 & HLA-A25 (MHC I)
Cellular localisation: Cell Surface - Molecular Weight
- ~41kDa
- Gene ID
- 3105, 181244, 654404
- UniProt
- P01889, P30443, P30499
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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