Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4) (AA 36-41) antibody

Catalog No. ABIN6937434

Product Details

Target: Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4)

Binding Specificity: AA 36-41

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant human HPV16 E1/E4 fragment (aa 36-41).

Clone: HPV16 E1-E4

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HPV-16 infected cells. Cervical tissue.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Human Papilloma Virus 16 E1, E4 (HPV-16 E1, E4)

Alternative Name: HPV16 E1/E4 (Human Papilloma Virus 16)

Target Type: Viral Protein

Background: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a 'high-risk' sexually-transmitted HPV that can lead to cervical, anal, vulvar, head, neck, and penile cancer. The HPV16E1/E4 protein is expressed abundantly in cells supporting viral DNA amplification, but is lost during malignant progression. HPV16E1/E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. HPV16E1/E4 also interacts with cyclin A and Cdk2 during the G2 phase of the cell cycle, and this association may increase the efficiency with which HPV16E1/E4 is able to prevent mitotic entry. HPV16E1/E4 also associates with keratin intermediate filaments and causes the network to collapse.

Molecular Weight: 16kDa