ICOSLG antibody (AA 23-149)

Catalog No. ABIN6937472

Product Details anti-ICOSLG Antibody

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Binding Specificity: AA 23-149

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICOSLG antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb recognizes a protein of 36 kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149) (exact sequence is proprietary)

Clone: ICOSL-3260

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HL60, HuT78 and HUVEC cells. Prostate or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: ICOSLG (ICOSLG Products)

Synonyms: ICOSLG antibody, AU044799 antibody, B7-H2 antibody, B7RP-1 antibody, B7h antibody, BG071784 antibody, GI50 antibody, GL50 antibody, GL50-B antibody, ICOS-L antibody, Icoslg antibody, LICOS antibody, Ly115l antibody, mKIAA0653 antibody, B7H2 antibody, B7RP1 antibody, CD275 antibody, ICOSL antibody, Icosl antibody, RGD1562791 antibody, inducible T-cell costimulator ligand antibody, icos ligand antibody, inducible T-cell co-stimulator ligand antibody, ICOSLG antibody, Icosl antibody, Icoslg antibody

Background: B7 homolog 2 (B7H2), B7 like protein Gl50, B7 related protein 1 (B7RP1), CD275, CD275 antigen, GL50, ICOS LG, ICOS ligand (ICOSL), Inducible T cell co stimulator ligand, LICOS, Transmembrane protein B7 H2 ICOS ligand,ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target)
Cellular localisation: Cell Surface

Molecular Weight: 36kDa

Gene ID: 23308, 14155

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation