Recombinant IGF1 antibody

Catalog No. ABIN6937488

The Rabbit Monoclonal anti-IGF1 antibody has been validated for WB, IHC, ELISA, IF, FACS and Coat. It is suitable to detect IGF1 in samples from Human.

Quick Overview for Recombinant IGF1 antibody (ABIN6937488)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Clone: IGF1-2872R

Product Details anti-IGF1 Antibody

Specificity: This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Cross-Reactivity (Details): Human. Mouse. Rat. Rabbit.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human IGF-1 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Breast, Thyroid or Colon Cancers (IHC). K562 cells (FACS). IGF-1 recombinant protein (WB).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1

Background: Insulin-like Growth Factor IA (IGF-IA), Insulin-like Growth Factor IB (IGF-IB), Mechano Growth Factor (MGF), Somatomedin-C,Insulin-like Growth Factor-1 (IGF-1)
Cellular localisation: Cytoplasmic (Secreted)

Molecular Weight: ~7.6kDa

Gene ID: 3479, 160562

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation