Recombinant IGKC antibody

Catalog No. ABIN6937539

Product Details anti-IGKC Antibody

Target: IGKC (Immunoglobulin kappa Constant (IGKC))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IGKC antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Ig kappa chain (IGKC) protein

Clone: KLC2289R

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IGKC

Target: IGKC (Immunoglobulin kappa Constant (IGKC))

Alternative Name: IGKC (IGKC Products)

Synonyms: HCAK1 antibody, IGKCD antibody, Km antibody, IGKC antibody, Igk-C antibody, immunoglobulin kappa constant antibody, immunoglobulin kappa constant L homeolog antibody, IGKC antibody, LOC708048 antibody, igkc antibody, igkc.L antibody, Igkc antibody

Background: HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM,Kappa Light Chain / IGKC (B-Cell Marker)
Cellular localisation: Cell Surface, cytoplasmic and secreted

Molecular Weight: ~22.5kDa

Gene ID: 3514, 449609

UniProt: P01601, P01834