C-JUN antibody (pThr91, pThr93)

Catalog No. ABIN6937611

The Mouse Monoclonal anti-C-JUN antibody is suitable to detect C-JUN in samples from Human and Rat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for C-JUN antibody (pThr91, pThr93) (ABIN6937611)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: C-J 4C4-1

Product Details anti-C-JUN Antibody

Binding Specificity: pThr91, pThr93

Purpose: Phospho C-Jun (Thr91, Thr93) Antibody

Specificity: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Cross-Reactivity (Details): Human. Rat.

Purification: 200ug/ml of Ab Purified by Protein A.

Immunogen: c-Jun protein phosphorylated at T91 and T93

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293, HeLA or A-431 cells, Human normal lung or bladder tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: JUN

Background:

Activator protein 1(API), cJun, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, Jun proto oncogene, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, VV jun sarcoma virus 17 oncogene homolog, V-jun avian sarcoma virus 17 oncogene homolog, vJun Avian Sarcoma Virus 17 Oncogene Homolog

HEK293, HeLA or A-431 cells, Human normal lung or bladder tissue.

Molecular Weight: 39kDa

Gene ID: 3725, 696684

UniProt: P05412

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins