KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18 antibody
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- Target
- KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
- Cross-Reactivity (Details)
- Human. Cow. Rat. Mouse. Guinea Pig. Frog. Goat. Marmoset. Pig.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Keratin-enriched preparation from cultured human A431
- Clone
- C11
- Isotype
- IgG1 kappa
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anti-KRT4, KRT5, KRT6, KRT8, KRT10, KRT13, KRT18 antibody
Reactivity: Human, Cow, Frog, Goat, Guinea Pig, Marmoset, Mouse, Pig, Rat FACS, IF, WB, IHC, StM Host: Mouse Monoclonal C11 unconjugated
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-4 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or A431 cells. Skin or colon carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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