LAG3 antibody

Catalog No. ABIN6937766

Product Details anti-LAG3 Antibody

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Coating (Coat)

Specificity: LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human LAG3 protein

Clone: LAG3-3261

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil or Hodgkin's lymphoma (IHC-P).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3 (LAG3 Products)

Synonyms: CD223 antibody, LAG-3 antibody, Ly66 antibody, lymphocyte activating 3 antibody, lymphocyte-activation gene 3 antibody, LAG3 antibody, Lag3 antibody

Background: CD223, FDC protein, LAG-3,Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)
Cellular localisation: Membrane.

Molecular Weight: 70kDa

Gene ID: 3902, 409523

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints