Moesin antibody

Catalog No. ABIN6937913

Product Details anti-Moesin Antibody

Target: Moesin (MSN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Human. Does not react with rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Moesin protein

Clone: MSN-492

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: K562, HeLa or HUVEC cells. Human uterus, placenta or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: MSN (MSN Products)

Synonyms: CG10701 antibody, D17 antibody, Dmel\\CG10701 antibody, Dmoe antibody, EMR1 antibody, ERM antibody, Emr1 antibody, anon-WO03040301.155 antibody, anon-WO03040301.157 antibody, anon-WO03040301.159 antibody, anon-WO03040301.161 antibody, dMoe antibody, gma antibody, l(1)G0067 antibody, l(1)G0323 antibody, l(1)G0404 antibody, l(1)G0415 antibody, moe antibody, moesin/ezrin/radixin homolog mRNA antibody, cb567 antibody, rdx antibody, wu:fb14b03 antibody, zgc:85640 antibody, MSN antibody, C78546 antibody, moesin antibody, Moesin antibody, moesin a antibody, moesin L homeolog antibody, MSN antibody, Moe antibody, msna antibody, msn.L antibody, moes antibody, Msn antibody

Background: Membrane-organizing extension spike protein, Moesin/anaplastic lymphoma kinase fusion protein, MSN/ALK fusion,Moesin
Cellular localisation: Cytoplasmic & Cell Surface

Molecular Weight: 78kDa

Gene ID: 4478, 87752

UniProt: P26038

Pathways: Asymmetric Protein Localization