Recombinant s100a4 antibody (AA 1-200)
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- Target See all s100a4 (S100A4) Antibodies
- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 1-200
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This s100a4 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
- Cross-Reactivity (Details)
- Human. Mouse.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary)
- Clone
- S100A4-2750R
- Isotype
- IgG
- Top Product
- Discover our top product S100A4 Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, A549 or A375 cells. Placenta.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
- Alternative Name
- S100A4 (S100A4 Products)
- Synonyms
- 18A2 antibody, 42A antibody, CAPL antibody, FSP1 antibody, MTS1 antibody, P9KA antibody, PEL98 antibody, 42a antibody, Capl antibody, FSp1 antibody, Mts1 antibody, PeL98 antibody, metastasin antibody, pk9a antibody, S100A4 antibody, P9ka antibody, RNP9KA antibody, S100 calcium binding protein A4 antibody, S100 calcium-binding protein A4 antibody, S100A4 antibody, S100a4 antibody
- Background
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S100A4, S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein,S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)
Cellular localisation: Cytoplasmic and Nuclear - Molecular Weight
- 10-12kDa
- Gene ID
- 6275, 654444
- UniProt
- P26447
- Pathways
- S100 Proteins
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