SERPINA3 antibody (AA 49-187)

Catalog No. ABIN6938377

Product Details anti-SERPINA3 Antibody

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Binding Specificity: AA 49-187

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SERPINA3 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: It recognizes a protein of 65-76 kDa, which is identified antichymotrypsin (AACT). AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of i± 1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants, their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Antichymotrypsin (AACT) protein fragment (around aa 49-187) (exact sequence is proprietary)

Clone: AACT-1451

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human tonsil, pancreas or histiocytoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SERPINA3

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Alternative Name: SERPINA3 (SERPINA3 Products)

Synonyms: SERPINA3-1 antibody, Pi2 antibody, AACT antibody, ACT antibody, GIG25 antibody, A1AT antibody, PI antibody, alpha-1-PI antibody, AAT antibody, Pi antibody, Spi1 antibody, SERPINA3-5 antibody, serpina3 antibody, serpin A3-5 antibody, serpin family A member 3 antibody, alpha-1-antiproteinase F precursor antibody, serpin family A member 1 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 L homeolog antibody, LOC396684 antibody, SERPINA3 antibody, LOC100008973 antibody, SERPINA1 antibody, Serpina1 antibody, SPIA5 antibody, serpina3.L antibody

Background: SERPINA3, AACT, ACT, Cell growth-inhibiting gene 24/25 protein, GIG24, GIG25, Growth inhibiting protein 24, Growth inhibiting protein 25, Serine (or cysteine) proteinase inhibitor clade A member 3, Serpin A3, Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3,Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker)
Cellular localisation: Cytoplasm

Molecular Weight: 65-76kDa

Gene ID: 12, 534293, 710488

UniProt: P01011