SUMO2 antibody

Catalog No. ABIN6938469

Product Details anti-SUMO2 Antibody

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUMO2 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.

Cross-Reactivity (Details): Human. Rat. Predicted to show a broad species reactivity.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human SUMO2 protein

Clone: SUMO2-1199

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Breast carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SUMO2

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Alternative Name: SUMO2 (SUMO2 Products)

Synonyms: HSMT3 antibody, SMT3B antibody, SMT3H2 antibody, SUMO3 antibody, Smt3A antibody, SMT3A antibody, SMT3H1 antibody, SUMO-3 antibody, Smt3B antibody, Smt3h2 antibody, sumo2 antibody, zgc:92241 antibody, SUMO2 antibody, SUMO-2 antibody, Smt3b antibody, hsmt3 antibody, smt3b antibody, smt3h2 antibody, sumo-2 antibody, PSMT3 antibody, ATSUMO2 antibody, MCO15.11 antibody, MCO15_11 antibody, SMALL UBIQUITIN-LIKE MODIFIER 2 antibody, SUM2 antibody, SUMO 2 antibody, small ubiquitin-like modifier 2 antibody, small ubiquitin-like modifier 2 antibody, small ubiquitin-like modifier 3 antibody, small ubiquitin-like modifier 2b antibody, small ubiquitin-related modifier 2 antibody, small ubiquitin-like modifier 2 S homeolog antibody, small ubiquitin-like modifier 2 L homeolog antibody, SUMO2 antibody, SUMO3 antibody, Sumo2 antibody, sumo2b antibody, LOC714892 antibody, sumo2.S antibody, sumo2.L antibody

Background: HSMT3, Sentrin 2, Small ubiquitin like modifier 2, Small ubiquitin like modifier protein 3, SMT3A, SMT3B, SMT3 homolog 1 (SMT3H1), SMT3 homolog 2 (SMT3H2), SMT3 homolog, SMT3 suppressor of mif two 3 homolog 1, SMT3 suppressor of mif two 3 homolog 2, SMT3 suppressor of mif two 3 homolog 3, Suppressor of mif two 3 homolog 2, Suppressor of mif two 3 homolog 3, Ubiquitin like protein SMT3A, Ubiquitin like protein SMT3B,SUMO-2
Cellular localisation: Nuclear

Molecular Weight: 11-13kDa

Gene ID: 6613, 474005

UniProt: P55854

Pathways: Methionine Biosynthetic Process