Transgelin antibody

Catalog No. ABIN6938511

Product Details anti-Transgelin Antibody

Target: Transgelin (TAGLN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transgelin antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Cross-Reactivity (Details): Human. Cow. Pig. Rabbit. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

Clone: TAGLN-247

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: TAGLN (TAGLN Products)

Synonyms: MGC68550 antibody, sm22 antibody, smcc antibody, tagln1 antibody, ws3-10 antibody, MGC107855 antibody, SM22 antibody, SMCC antibody, TAGLN1 antibody, WS3-10 antibody, Sm22 antibody, Sm22a antibody, Ws310 antibody, SM22A antibody, zgc:153186 antibody, transgelin L homeolog antibody, transgelin antibody, tagln.L antibody, tagln antibody, LOC732906 antibody, TAGLN antibody, tagl antibody, LOC100009332 antibody, Tagln antibody

Background: 2 kDa actin-binding protein, Human 22 kDa smooth muscle protein, Protein WS3-10, SMCC, Smooth muscle protein 22-alpha (SM22-alpha), TAGLN, TAGLN1, transgelin, Transgelin variant 2, WS3-10,Transgelin (SM22-alpha)
Cellular localisation: Cytoplasmic

Molecular Weight: 22kDa

Gene ID: 6876, 410977

UniProt: Q01995