TGFA antibody (C-Term)
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- Target See all TGFA Antibodies
- TGFA (Transforming Growth Factor, alpha (TGFA))
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Binding Specificity
- C-Term
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TGFA antibody is un-conjugated
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Application
- Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)
- Specificity
- This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
- Cross-Reactivity (Details)
- Human. Cow. Rat. Mouse.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)
- Clone
- MF9
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TGFA Primary Antibody
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- Application Notes
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat or Ramos cells. Testicular Carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- TGFA (Transforming Growth Factor, alpha (TGFA))
- Alternative Name
- TGFA (TGFA Products)
- Synonyms
- TFGA antibody, LOC100009482 antibody, TGF alpha antibody, TGFA antibody, wa-1 antibody, wa1 antibody, RATTGFAA antibody, TGFAA antibody, NV15606 antibody, si:ch211-10e8.7 antibody, transforming growth factor alpha antibody, transforming growth factor alpha L homeolog antibody, transforming growth factor-alpha antibody, transforming growth factor, alpha antibody, TGFA antibody, TGF-A antibody, Tgfa antibody, tgfa.L antibody, LOC100121300 antibody, LOC100009150 antibody, tgfa antibody
- Background
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EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1,TGF-alpha (Transforming Growth Factor alpha)
Cellular localisation: Cytoplasmic and Extracellular (secreted) - Molecular Weight
- ~6kDa
- Gene ID
- 7039, 170009
- UniProt
- P01135
- Pathways
- NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway
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