Recombinant Thrombomodulin antibody (AA 69-194)

Catalog No. ABIN6938551

Product Details anti-Thrombomodulin Antibody

Target: Thrombomodulin (THBD)

Antibody Type: Recombinant Antibody

Binding Specificity: AA 69-194

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)

Clone: RTHBD-1591

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: THP1 cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: THBD (THBD Products)

Synonyms: AHUS6 antibody, BDCA3 antibody, CD141 antibody, THPH12 antibody, THRM antibody, TM antibody, AI385582 antibody, THBD antibody, HAST antibody, HAST3 antibody, M-PST antibody, ST1A3/ST1A4 antibody, ST1A5 antibody, STM antibody, TL-PST antibody, thrombomodulin antibody, sulfotransferase family 1A member 3 antibody, THBD antibody, Thbd antibody, SULT1A3 antibody

Background: AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM),Thrombomodulin / CD141 (Endothelial Cell Marker)
Cellular localisation: Cell Surface & Cytoplasmic

Molecular Weight: ~100kDa

Gene ID: 7056, 2030

UniProt: P07204