VISTA antibody
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- Target See all VISTA Antibodies
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This VISTA antibody is un-conjugated
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Application
- ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human VISTA protein
- Clone
- VISTA-3007
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product VISTA Primary Antibody
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- Application Notes
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Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Spleen or Placenta.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
- Alternative Name
- VSIR (VISTA Products)
- Synonyms
- MGC112715 antibody, MGC151567 antibody, B7-H5 antibody, B7H5 antibody, GI24 antibody, PP2135 antibody, SISP1 antibody, Dies1 antibody, VISTA antibody, V-set immunoregulatory receptor antibody, vsir antibody, Vsir antibody, VSIR antibody
- Background
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B7H5, DD1alpha, GI24, PD-1H, PDCD1 homolog, Platelet receptor Gi24, PP2135, Stress-induced secreted protein 1 (SISP1), V domain Ig suppressor of T cell activation (VISTA), V Set Immuno-Regulatory Receptor,VISTA / B7-H5 / VSIR (Negative Regulator of Immune Response)
Cellular localisation: Cell Surface and Cytoplasmic - Molecular Weight
- 34kDa
- Gene ID
- 64115, 47382
- UniProt
- Q9H7M9
- Pathways
- Cancer Immune Checkpoints
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