SEMA4D/CD100 antibody
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- Target See all SEMA4D/CD100 (SEMA4D) Antibodies
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SEMA4D/CD100 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)
- Specificity
- Recognizes a homodimeric protein comprised of 50 kDa subunits, identified as CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.
- Cross-Reactivity (Details)
- Human, Monkey, Mouse,
- Purification
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
- Immunogen
- PHA stimulated human peripheral blood lymphocytes
- Clone
- A8
- Isotype
- IgG1
- Top Product
- Discover our top product SEMA4D Primary Antibody
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- Application Notes
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Positive Control: Daudi, Raji, HUT-78, Kg1a, U937 and human lymphocytes. Human tonsils and lymph nodes.
Known Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
- Expiry Date
- 24 months
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- Target
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
- Alternative Name
- SEMA4D (SEMA4D Products)
- Synonyms
- C9orf164 antibody, CD100 antibody, M-sema-G antibody, SEMAJ antibody, coll-4 antibody, Semacl2 antibody, Semaj antibody, Semcl2 antibody, COLL-4 antibody, semaphorin 4D antibody, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D antibody, Sema4d antibody, SEMA4D antibody
- Background
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COLL4, Collapsin4, GR3, M sema G, MSEMA, SEMA4D, Semacl2, SemaH, SEMAJ, Semaphorin C like 2, Semaphorin H, Semaphorin J, Semaphorin-4D, Semcl2,CD100 (Semaphorin-4D)
Cellular localisation: Cell surface - Molecular Weight
- 50kDa (Monomer)
- Gene ID
- 10507, 494406
- UniProt
- Q92854
- Pathways
- Regulation of Cell Size
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