FAF1 antibody

Catalog No. ABIN6935809

Product Details anti-FAF1 Antibody

Target: FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved 'death domain' and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-RI interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-RI-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-RI via their DDH regions, while RIP associates exclusively with FAS. An additional FAS interacting protein designated FAF1, for FAS-associated protein factor-1, binds with the cytoplasmic tail of wildtype but not LPR mutant FAS. When overexpressed in cells, FAF1 enhances the efficiency of FAS-mediated apoptosis. In contrast to TRADD, FADD and RIP, FAF1 lacks a DDH and cannot induce apoptosis independently of FAS activation.

Cross-Reactivity (Details): Human,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length FAF1 protein

Clone: CPTC-FAF1-2

Isotype: IgG2b

Application Details

Application Notes:

Positive Control: HEK293, MCF-7, HeLa whole cell lysate. Human testis, prostate, breast carcinoma or colon carcinoma.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for FAF1

Target: FAF1 (Fas (TNFRSF6) Associated Factor 1 (FAF1))

Alternative Name: FAF1 (FAF1 Products)

Synonyms: faf1 antibody, MGC53741 antibody, MGC80303 antibody, FAF1 antibody, zgc:55752 antibody, wu:fc25h12 antibody, HFAF1s antibody, UBXD12 antibody, UBXN3A antibody, hFAF1 antibody, AA408698 antibody, Dffrx antibody, Fam antibody, Fas (TNFRSF6) associated factor 1 S homeolog antibody, Fas (TNFRSF6) associated factor 1 L homeolog antibody, Fas associated factor 1 antibody, Fas (TNFRSF6) associated factor 1 antibody, Fas-associated factor 1 antibody, faf1.S antibody, faf1.L antibody, FAF1 antibody, faf1 antibody, Faf1 antibody

Background: CGI-03, FAS-associated factor 1, HFAF1s, UBX domain-containing protein 12, UBX domain-containing protein 3A,Fas (TNFRSF6) associated factor 1
Cellular localisation: Nucleus.

Molecular Weight: 75-80kDa

Gene ID: 11124, 530402

UniProt: Q9UNN5

Pathways: Maintenance of Protein Location