alpha Fetoprotein antibody
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- Target See all alpha Fetoprotein (AFP) Antibodies
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This alpha Fetoprotein antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.
- Purification
- Purified by Protein A/G
- Immunogen
- Human Alpha feto protein
- Clone
- MBS-12
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product AFP Primary Antibody
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- Application Notes
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Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- Alternative Name
- AFP (AFP Products)
- Synonyms
- FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
- Molecular Weight
- 70kDa
- Gene ID
- 174
- UniProt
- P02771
- Pathways
- C21-Steroid Hormone Metabolic Process
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