Recombinant Histone H1 antibody
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- Target See all Histone H1 Antibodies
- Histone H1
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Histone H1 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Immunogen
- Nuclei of human leukemia biopsy cells
- Clone
- AE-4
- Isotype
- IgG
- Top Product
- Discover our top product Histone H1 Primary Antibody
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- Application Notes
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Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma (IHC).
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Histone H1
- Alternative Name
- Histone H1 (Pan Nuclear Marker) (Histone H1 Products)
- Synonyms
- H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody
- Background
- Please note that this antibody is a recombinant Rabbit version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.
- Molecular Weight
- ~30kDa
- Gene ID
- 3005
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