HLA-DRA antibody
-
- Target See all HLA-DRA Antibodies
- HLA-DRA (HLA Class II DR alpha (HLA-DRA))
-
Reactivity
- Human, Monkey
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This HLA-DRA antibody is un-conjugated
-
Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50 % of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70 % of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. '
- Purification
- Purified by Protein A/G
- Immunogen
- Spleen cells of a patient with hairy cell leukemia (Daudi cells)
- Clone
- IPO-10
- Isotype
- IgG3 kappa
- Top Product
- Discover our top product HLA-DRA Primary Antibody
-
-
- Application Notes
-
Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- HLA-DRA (HLA Class II DR alpha (HLA-DRA))
- Alternative Name
- HLA-DRA (HLA-DRA Products)
- Synonyms
- HLA-DRA1 antibody, MLRW antibody, DR-alpha antibody, HLA-DRA antibody, Mamu-DRA1 antibody, major histocompatibility complex, class II, DR alpha L homeolog antibody, major histocompatibility complex, class II, DR alpha antibody, hla-dra.L antibody, HLA-DRA antibody, MAMU-DRA antibody, BOLA-DRA antibody
- Molecular Weight
- ~36kDa
- Gene ID
- 3122
- UniProt
- P01903
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events
-