IGF1 antibody

Catalog No. ABIN6939700

The Mouse Monoclonal anti-IGF1 antibody is suitable to detect IGF1 in samples from Human, Mouse, Rat and Rabbit. It has been validated for IHC, ELISA, FACS and IF.

Quick Overview for IGF1 antibody (ABIN6939700)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPM406

Product Details anti-IGF1 Antibody

Purpose: Insulin-like Growth Factor-1 (IGF-1) Antibody

Specificity: This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified human IGF-1 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers, IGF-1 recombinant protein.

Known Application: ELISA (For coating, purchase antibody without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT), In-vitro Neutralization of Biological Activity of IGF-1 (Order Ab without Azide)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1

Background:

Insulin-like Growth Factor IA (IGF-IA), Insulin-like Growth Factor IB (IGF-IB), Mechano Growth Factor (MGF), Somatomedin-C

Pancreas or brain. Breast, Thyroid or Colon Cancers, IGF-1 recombinant protein.

Molecular Weight: ~7.6kDa

Gene ID: 3479, 160562

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation