MDM2 antibody (AA 154-167)

Catalog No. ABIN6940030

This Mouse Monoclonal antibody specifically detects MDM2 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MDM2 antibody (AA 154-167) (ABIN6940030)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: SMP14

Product Details anti-MDM2 Antibody

Binding Specificity: AA 154-167

Purpose: MDM2 Antibody

Specificity: MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53. It can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Overexpression of MDM2 protein is detected in a variety of cancers.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide (aa 154-167) of human MDM2 protein.

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HepG2 cells. Breast Carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2

Background:

ACTFS, Double minute 2 protein, E3 ubiquitin-protein ligase, Hdm2, HDMX, MDM2, MDM2 oncogene E3 ubiquitin protein ligase, MDM2BP, Mouse Double Minute 2, MTBP, Murine Double Minute Chromosome 2, p53 Binding Protein Mdm2, p53-binding protein Mdm2

HepG2 cells. Breast Carcinoma.

Molecular Weight: ~90kDa

Gene ID: 4193, 484551

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway