PCNA antibody

Catalog No. ABIN6940267

This Mouse Monoclonal antibody specifically detects PCNA in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat and Saccharomyces cerevisiae.

Quick Overview for PCNA antibody (ABIN6940267)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: PCNA-3065

Product Details anti-PCNA Antibody

Purpose: PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Specificity: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Rat PCNA/Protein A fusion protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Tonsil or reactive lymph node. MOLT-4, HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

HepG2, THP-1, A-431, NIH/3T3, A-549, SKBr3, HEK293 or U937 cells, Human Tonsil or Human Colon

Molecular Weight: 36kDa

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy