Retinoblastoma 1 antibody

Catalog No. ABIN6940479

This Mouse Monoclonal antibody specifically detects Retinoblastoma 1 in WB, IHC, IP and StM. It exhibits reactivity toward Human and Mouse.

Quick Overview for Retinoblastoma 1 antibody (ABIN6940479)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Staining Methods (StM)

Clone: 1F8

Product Details anti-Retinoblastoma 1 Antibody

Specificity: Recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Purification: Purified by Protein A/G

Immunogen: Recombinant human Rb1 protein fragment of 283 amino acid residues.

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.

Known Application: Western Blot (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein lysate),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: RB1

Molecular Weight: 105kDa

Gene ID: 5925

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy