Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN6940518

The Mouse Monoclonal anti-Smooth Muscle Actin antibody is suitable to detect Smooth Muscle Actin in samples from Human, Rat, Mouse, Chicken, Rabbit, Cow, Monkey, Guinea Pig, Baboon, Pig, Dog, Sheep, Goat and Cat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN6940518)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Cow, Monkey, Guinea Pig, Baboon, Pig, Dog, Sheep, Goat, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 1A4-same as asm-1

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Purpose: Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody

Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: HeLa cells (FACS/IF). Blood vessels in all tissues, smooth muscle or leiomyosarcoma (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: ACTA2

Background:

ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

HeLa cells, smooth muscle or leiomyosarcoma, Mouse 3T3 cells

Molecular Weight: 42kDa

Gene ID: 59, 500483

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development