CD4 antibody

Catalog No. ABIN6941039

The Mouse Monoclonal anti-CD4 antibody is suitable to detect CD4 in samples from Human, Macaca arctoides, Chimpanzee and Orang-Utan. It has been validated for FACS, IF and Func.

Quick Overview for CD4 antibody (ABIN6941039)

Target: CD4

Reactivity: Human, Macaca arctoides, Chimpanzee, Orang-Utan

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: EDU-2

Product Details anti-CD4 Antibody

Purpose: CD4 (T-Helper/Inducer Cell Marker) Antibody

Specificity: Recognizes a protein of 55 kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.This MAb was characterized as human CD4 antibody at II and IV International Workshop on Human Leukocyte Differentiation Antigens.

No Cross-Reactivity: Rhesus Monkey

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60. Lymph node or tonsil.

Known Application: Functional Studies (Order Ab without azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background:

L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

CCRF-CEM or HL-60. Lymph node or tonsil., Human peripheral blood mononuclear cells (PBMCs)

Molecular Weight: 55kDa

Gene ID: 920, 631659

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events