CD4 antibody

Catalog No. ABIN6941040

The Mouse Monoclonal anti-CD4 antibody (Clone RIV7) (ABIN6941040) specifically detects CD4 in FACS, IF and Func. The antibody is reactive with Human and Monkey samples.

Quick Overview for CD4 antibody (ABIN6941040)

Target: CD4

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: RIV7

Product Details anti-CD4 Antibody

Purpose: CD4 (T-Helper/Inducer Cell Marker) Antibody

Specificity: Recognizes a protein of 55 kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human peripheral lymphocytes

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60. Lymph node or tonsil.

Known Application: Functional Studies (Order Ab without azide), Flow Cytometry (1-2 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL) Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background:

L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

CCRF-CEM or HL-60. Lymph node or tonsil., Human peripheral blood mononuclear cells (PBMCs)

Molecular Weight: 55kDa

Gene ID: 920, 631659

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events