G1/S-Specific B-Type Cyclin Cig2 (CIG2) antibody
CIG2
Reactivity: Schizosaccharomyces pombe
Coat, ELISA, WB
Host: Mouse
Monoclonal
3A11-5
unconjugated
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- Target See all G1/S-Specific B-Type Cyclin Cig2 (CIG2) products
- G1/S-Specific B-Type Cyclin Cig2 (CIG2)
- Reactivity
- Schizosaccharomyces pombe
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Coating (Coat), ELISA, Western Blotting (WB)
- Purification
- Purified by Protein A/G
- Immunogen
- Bacterial S. pombe Cig2 Purified protein.
- Clone
- 3A11-5
- Isotype
- IgG1 kappa
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- Application Notes
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Positive Control: Red blood cell lysate, Human colon and lung tissue.
Known Application: ELISA (For coating, order antibody without BSA),Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- G1/S-Specific B-Type Cyclin Cig2 (CIG2)
- Alternative Name
- Cig2 (CIG2 Products)
- Synonyms
- G1/S-specific B-type cyclin Cig2 antibody, cig2 antibody
- Background
- Cig2 is a cyclin that functions as the catalytic subunit of cyclin-dependent kinases (Cdks) in the fission yeast Schizosaccharomyces pombe. It is a B-type, S phase cyclin that is required for both the G1/S and G2/M cell cycle transitions and is expressed in a sharp spike that peaks during the G1/S period. Cig2 binds to Cdc2 (Cdk), and the resulting Cdc2/Cig2 complex controls the G1/S transition of the cell cycle. Disruption of Cig2 delays the onset of mitosis. The expression of the Cig2 gene is dependent on Mlu1-binding factor (MBF), and the protein is destroyed during anaphase by the APC/cyclosome (APC/C) and Skp1/Cullin-1/F-box (SCF), thereby ensuring the spike expression pattern of Cig2. SCF regulates Cig2 levels in a dual manner, transcriptionally and post-translationally, while APC/C only destroys the protein.
- Molecular Weight
- 50kDa
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